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--- algae_generator [2019/08/01 17:43] – [Growing algae] ami
+++ algae_generator [2019/11/10 23:07] (current) – [DIY bio-Experiments] ami
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 ======ALGAE for a Sensorial Skin======
-At the core of this project is too research a biosensor to measure pollution in urgan areas (the foraging fields around the beehive).
+At the core of this project is too research a biosensor to measure pollution in urban areas (the foraging fields around the beehive).
-A take a naturalist approach to start this project: a naturalist is a type of biologist who studies the impacts of living species on each other and the environments in which they live. I will study the different elements of the rooftop garden and their relation to each other, to get a better insight from the links of this mini-ecosystem: the bees, the plants, the soil, the bacteria, the lichens, the a-biotic elements as minerals, air composition, gasses, temperature etc...
+A take a naturalist approach to start this project: a naturalist is a type of biologist who studies the impacts of living species on each other and the environments in which they live. I study the different elements of the rooftop garden and how they relate to each other, to get a better insight in the dynamic network of this mini-ecosystem: the bees, the plants, the soil, the bacteria, the lichens, the a-biotic elements as minerals, air composition, gasses, temperature etc...
-Naturalism is the study of the natural world as a whole and the study of each species within its environment, as well as how species interact with each other. A naturalist will look for evolutionary markers to explain why a certain attribute may have been beneficial or what environmental conditions may have led to the rise of genetic diversity within a species. The naturalists' understanding is in ecology, natural selection, sexual selection and environmental forcings.
+Naturalism is the study of the natural world as a whole and the study of each species within its environment, as well as how species interact with each other. Evolutionary science is at the core of the naturalists' work, which is very much about observations in the field next to work in the lab. Therefore I spent a lot of time in my rooftopgarden-openair-laboratory, taking field notes, photographs and written records and I am compiling them for analysis.
-Evolutionary science is at the core of the naturalists' work, which is all about observations in the field rather than in the lab. The typical naturalist will spend most of his time in nature rather than in labs, taking field notes, photographs and written records and compiling them for analysis.
 {{:algae-generator:algae-2.jpg?600|}}
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+=====Air Pollution=====
+Air pollution is characterized by the presence of gas and particles in the outside air with harmful impact on human health and/or on the environment. Those pollutants come from natural phenomena (volcanic eruption, organic matter decomposition, forest fire) as well as human activity (industry, transport, farming and residential heating). This Particulate Matter (PM) is a combination of liquid droplets and solid particles. Some are visible with the naked eye e.g. smoke, dust, etc. Others cannot be seen without visual aids such as a microscope. These particles come in all shapes, forms and sizes and can consist out of a plethora of different chemicals. This indicates that they can come from many different sources either direct or indirect. Examples of such sources are car exhaust gases, agriculture, road transport, construction sites, pollutants from industries and power plants… (non-limited list).
+http://bioinformatics.biobix.be/aerolis/notebook.html#mo
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 Synechocystis is a genus of unicellular, freshwater cyanobacteria in the family Merismopediaceae. It includes a strain, Synechocystis sp. PCC 6803, which is a well studied model organism. (wikipedia)
 Besteld op 08/07/2019 via Open BioLab: {{ :algae-generator:bestellingen_micro-algae.pdf |}}
-{{gallery>:algae-generator:growing-algae}}
 Gekregen van Maria Boto in maart 2019: Spirulina.
 Geoogst in de Laguna op La Certosa, Venezia: Spirogyra
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 [[http://aqualgae.com/en/productos-y-servicios/strains-and-starter-cultures/|algae starter cultures]]
 [[http://bccm.belspo.be/about-us/bccm-lmg|Belgian Collections of Micro-organisms]]
-https://utex.org/pages/search-results?category_id=30137088&category_url=%2Fcollections%2Fliving-algal-strains&page_num=2
 https://algaeresearchsupply.com/collections/algae-cultures
 https://algaecentrifuge.com/products/algae-culture-kit
 https://www.carolina.com/algae/algae-culture-kit/151500.pr
+{{gallery>:algae-generator:growing-algae}}
 Monitoring the algae growth: parameters:
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-{{gallery>:algae-generator:growing_synechocystis}}
 **Long Term Culturing**
 Agar-based media are best for long-term culturing. Bold's basic agar with proteose peptone added does well for many of the green algae, and Allen’s modified blue-green agar supports a wide range of blue-green algae (cyanobacteria). One disadvantage of using agar for long-term cultures is that the cultures must be axenic (bacteria-free). For bacterized unialgal cultures and those that will not grow on agar, Soil–Water Medium or one of its variants is good. Another way to make a good long-term medium is to add a layer of liquid (freshwater or seawater) over agar that contains the appropriate nutrients.
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 {{gallery>:sensorial-skin:algae-exp1}}
 **Experiment 1**: how to keep up water on the surface of the skin, so that the algae can grow?
+Design of skin-shells, based upon pollen textures or leaf textures (to keep moist up).
+The idea is to print (3D print?) these textures as a flexible, hollow jacket/bag that can be clad around the torso of the beehive. The bag would than be filled with photosynthesising micro-algae that provide energy for the sensors in the beehive:
+{{gallery>:sensorial-skin:algae-exp2}}
 Membrane-based architectures enable optimization of charge transport and electrochemical potential gradients in artificial photosynthesis. https://www.researchgate.net/publication/317032127_Membranes_for_artificial_photosynthesis
 [[https://www.google.com/search?q=artificial+photosynthesis+and+CNT+embedded+polymer+film&client=firefox-b-ab&tbm=isch&tbo=u&source=univ&sa=X&ved=2ahUKEwjcmeD3__feAhWqAMAKHYTtCU8QsAR6BAgFEAE&biw=1258&bih=726|artificial photosynthesis and CNT embedded polymer film]]
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 [[https://inhabitat.com/new-3d-printed-algae-could-revolutionize-the-way-we-make-things/antoineraab/|studio Luma]]
 [[http://cargocollective.com/irina_shaklova/Living-Screen|algae print with hydrogel, Kukarobot]]
-{{gallery>:sensorial-skin:algae-exp2}}
+[[https://issuu.com/irinashaklova/docs/thesis_irina_shaklova_cor_37ec46bbb3705e|algal screen - IAAC]]
+[[https://en.wikipedia.org/wiki/Hydrogel|Hydrogel]]
+{{ :algae-generator:algae_hydrogel.pdf |Algae printed in Hydrogel}}
-**Experiment 2**: design of skin-shells, based upon pollen textures or leaf textures.
-The idea is to print (3D print?) these textures as a flexible, hollow jacket/bag that can be clad around the torso of the beehive. The bag would than be filled with photosynthesising micro-algae that provide energy for the sensors in the beehive:
-{{gallery>:sensorial-skin:algae-exp3}}
-**Experiment 3**: Grow Cyanobacteria on casted object (started 25/7/2019)
+**Experiment 2**: Grow Cyanobacteria on casted objects (started 25/7/2019)
 {{gallery>:algae-generator:bacteria_objects}}
 Processbased artwork. The object is an epoxy cast of a 3D print, based on the design of a bacteria. I wrapped it in microbial skin and I inoculated the skin wih cyanobacteria. All is in a sealed box with soil-percolate at the bottom (in the black sand). I hope that something will grow on the 3D structure. Maybe just stains of cyanobacteria or maybe it will be mold, as this set-up was not sterilized.
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 This will be the last research-part of our project.
 [[https://www.nemokennislink.nl/publicaties/crispr-cas-is-perfect-om-erfelijke-aandoeningen-te-genezen/?news_letter=true&utm_medium=email&utm_campaign=Nieuwsbrief%2026%20juli%202019%20-%20Wat%20veroorzaakt%20extreme%20verzameldrang&utm_content=Nieuwsbrief%2026%20juli%202019%20-%20Wat%20veroorzaakt%20extreme%20verzameldrang+CID_5d0a20bb91972e2a666c523597899263&utm_source=Email%20marketing%20software&utm_term=CRISPR-Cas%20is%20perfect%20om%20erfelijke%20aandoeningen%20te%20genezen|CRISPR-cas]]
+[[http://www.inbio.ugent.be/staff_MaDeme.htm|Professor Marjan Demey, UGent,  Metabolic Engineering of Microorganisms]]
+{{ :algae-generator:biofunction-aerolisproject.pdf |}}
+[[https://www.i3s.up.pt/research-group?x=5|Professor P.Tamagnini, University of Porto, Bioengineering & Synthetic Microbiology]]
+[[http://2016.igem.org/Team:Peshawar/Description|IGEM Air Pollution Sensor project]]
+{{:algae-generator:signaturebccm.jpg?400|Dr. Annick Wimotte, Université de Liège}}: My field of work is the biodiversity, phylogeny and taxonomy of cyanobacteria and I lead a public culture collection of cyanobacterial strains with a focus on Antarctic and Arctic ones. Note that as a culture collection, we are always interested by outreach activities with artists. We are also member of the Belgian Consortium of culture collections (BCCM) including bacteria and fungi.
 \\
 =====Microbial fuel cells, bio fuel cells=====
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 - {{ :algae-generator:leviathan_algarium_veneticuma_history_of_algae_and_autarchy.pdf |Algarium Veneticum}}
 - {{ :algae-generator:algariumveneticum_.pdf |Algarium Herbarium}}
+- [[https://www.labmakelaar.com/nld/detail/oplossen-roeren-en-schudden/schudmachines/9684-bioworld-ar100-rocker-platform/9684|labmateriaal 2de hands]]