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hydrophobic_bc

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HYDROPHOBIC BC

Iza Awad & AnneMarie Maes, Hybrid Forms Lab - VU Amsterdam

Research:

Trying to make BC waterproof with a coating of (cyano)bacteria.
In a first phase, we will work with Nostoc cyanobacteria.
In a second phase, we will add samples of lichen to the Nostoc impregnated BC.


Nostoc sp. op BC, in pellicule van agarose met algagrow (voorkant en achterkant)

Protocol

Nostoc:
BC Neutraliseren (20 g) met 1 M Na2CO3 (100x diluted, voeg steeds mL toe tot pH=7),
Geneutraliseerde BC 4x wassen met BG-11,
BC-BG11 autoclaveren (met of zonder BG medium, 20 mL) en UV licht (optioneel),
Opbrengen van de nostoc (triplo).
Incuberen op 20 graden celsius.
Elke dag de groei monitoren (microscopisch?)

Nostoc + Lichen:

BC Neutraliseren (20 g) met 1 M Na2CO3 (100x diluted, voeg steeds mL toe tot pH=7),
Geneutraliseerde BC 4x wassen met BG-11,
BC-BG11 autoclaveren (met of zonder BG medium, 20 mL) en UV licht (optioneel),
Opbrengen van de nostoc (triplo).
Verpulverde lichen (5 g. zonder aarde en schoon, wassen 5x met tap water) aanbrengen op nostoc-BC
Incuberen op 20 graden celsius.
Elke dag de groei monitoren (microscopisch?)

Brussels Tests

Test1 (Brussel, 25/2/2020): Nostoc; Nostoc + Lichen

Test1 (Brussel, 25/2/2020): Synechocystis; Synechocystis + Lichen

I followed the protocol we discussed,
- I took a nice piece of BC that I’ve cut in pieces of 20 grs each.
- I neutralised the BC as prescribed in the protocol, and I autoclaved the little pieces, together with a bit of BG11 still in the bottles.
- I’ve put the pieces in sterile petri dishes (working with the flame) and I inoculated them with Nostoc & Synechocystis (separately), on some petri's I added cleaned lichen on top of the cyanobacteria.
- Everytime I made 2 petri’s, to have a control.
- I’ve put the petri's in the incubator at 22°C, on 25/2/2020.
- When I came back from Paris and when I controlled the petri’s, there was the same mould on all BC pieces.
This kind of ‘greenish blue cheese penicilline mould’. On the samples inoculated with Synechocystis, there was besides the blue/green mould also a kind of black mould.
- The samples with cyanobacteria + lichen had more mould than the ones without lichen. The samples inoculated with Synechocystis had more mould than the samples with Nostoc. The little black pieces on top of the BC on the petri’s with ‘lichen’ samples, are the lichen.
- the contaminated samles are not yet tested on pH, neither I have inspected them under the microcope. This needs to be done asap.

hydrophobic_bc.1583746405.txt.gz · Last modified: 2020/03/09 10:33 by ami