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--- hydrophobic_bc [2020/02/13 14:10] – created ami
+++ hydrophobic_bc [2020/03/09 09:18] – [Brussels Tests] ami
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 ======HYDROPHOBIC BC======
+Iza Awad & AnneMarie Maes, Hybrid Forms Lab - VU Amsterdam
+=====Research:=====
+Trying to make BC waterproof with a coating of (cyano)bacteria.
+In a first phase, we will work with Nostoc cyanobacteria.
+In a second phase, we will add samples of lichen to the Nostoc impregnated BC.
+{{:hydrophobicbc:nostoc_bc_voorkant.jpeg?300|}} {{:hydrophobicbc:nostoc_bc_achterkant.jpeg?276|}}
+//Nostoc sp. op BC, in pellicule van agarose met algagrow (voorkant en achterkant)//
+=====Protocol=====
+__Nostoc:__
+BC Neutraliseren (20 g) met 1 M Na2CO3 (100x diluted, voeg steeds mL toe tot pH=7),
+Geneutraliseerde BC 4x wassen met BG-11,
+BC-BG11 autoclaveren (met of zonder BG medium, 20 mL) en UV licht (optioneel),
+Opbrengen van de nostoc (triplo).
+Incuberen op 20 graden celsius.
+Elke dag de groei monitoren (microscopisch?)
+__
+Nostoc + Lichen:__
+BC Neutraliseren (20 g) met 1 M Na2CO3 (100x diluted, voeg steeds mL toe tot pH=7),
+Geneutraliseerde BC 4x wassen met BG-11,
+BC-BG11 autoclaveren (met of zonder BG medium, 20 mL) en UV licht (optioneel),
+Opbrengen van de nostoc (triplo).
+Verpulverde lichen (5 g. zonder aarde en schoon, wassen 5x met tap water) aanbrengen op nostoc-BC
+Incuberen op 20 graden celsius.
+Elke dag de groei monitoren (microscopisch?)
+\\
+=====Brussels Tests=====
+Test1 (Brussel, 25/2/2020): Nostoc; Nostoc + Lichen
+{{gallery>:hydrophobicbc:test1brussel-nostoc}}
+Test1 (Brussel, 25/2/2020): Synechocystis; Synechocystis + Lichen
+{{gallery>:hydrophobicbc:test1brussel-synechocystis}}
+I followed the protocol we discussed,
+- I took a nice piece of BC that I’ve cut in pieces of 20 grs each.
+- I neutralised the BC as prescribed in the protocol, and I autoclaved the little pieces, together with a bit of BG11 still in the bottles.
+- I’ve put the pieces in sterile petri dishes (working with the flame) and I inoculated them with Nostoc & Synechocystis (separately), on some petri's I added cleaned lichen on top of the cyanobacteria.
+- Everytime I made 2 petri’s, to have a control.
+- I’ve put the petri's in the incubator at 22°C, on 25/2/2020.
+- When I came back from Paris and when I controlled the petri’s, there was the same mould on all BC pieces.
+This kind of ‘greenish blue cheese penicilline mould’. On the samples inoculated with Synechocystis, there was besides the blue/green mould also a kind of black mould.