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--- dyeing_with_bacteria_and_natural_dyes [2018/04/17 22:10] – [organic dyes] ami
+++ dyeing_with_bacteria_and_natural_dyes [2018/04/24 15:41] (current) – [organic dyes and bioplastics] annemie
@@ Line 1: / Line 1: @@
-=====dyeing with Bacteria and Natural Dyes=====
+=====dyeing Natural Dyes=====
 {{:dyeing:table.jpg?400x278|biolab collserola}} {{:dyeing:tabletop.jpg?400x277|tabletop: avocado dye and cochineal dye}}
 //My Lab at Collserola - dye workspace. Avocado dye and Cochineal dye.//
@@ Line 12: / Line 12: @@
 \\
-=====dyeing with Janthinobacterium lividum on microbial skins=====
+=====organic dyes=====
-{{gallery>:learning-from-nature:bacteria_skin}}
+{{gallery>:learning-from-nature:skin_dyes&lightbox}}
-LB Medium (250ml gedemineraliseerd water; 6,25gr LB powder; 4gr glycerine; mengen en autoclaven).
-Optimum growing conditions for Janthinobacterium: 25°C.
-Glycerine is enhancing the growth of Janthinobacterium; Glucose is inhibiting the growth.
-Bacteria on sterilized cellulose skin (grote petri-schalen): 1ste poging (met gesterelizeerde skins) is mislukt - kan ook liggen aan slecht groeimedium. 2de poging (LB met glycerine en nieuwe inoculatie van bacteria) is in wording.
-Nieuwe test met kleine petri schalen en kleine stukken cellulose skin: teststukjes cellulose skin zo 'steriel' mogelijk gemaakt zonder ze te autoclaven (heet water, houden boven stoom). Meeste vocht uit de skin laten opdrogen. Daarna de skins goed bevochtigen met LB medium mét glycerine, en inoculeren met Janthinobacterium lividum.
-Bacteria on sterilized white cotton fabric: katoen ge-autoclaved en bacteria onder steriele omstandigheden geinoculeerd. Na 2 dagen resultaat, blauwe tekening.
-=====workshop bioshades Barcelona - 15/3/2018=====
-{{url>https://www.youtube.com/embed/gJvxqujkUvI?rel=0#?embed=true 560px,315px noborder}}
+Madder => madder powder, solution in water, added to medium for growing skins. This growth medium is completely rejected by the scoby. No skin is formed, and the starter scoby's are eaten away by the solution.
-http://docs.academany.org/softacademy-handbook/_book/classes/00_intro.html
+Alkanet => alkanet seeds extracted in ethanol, solution added to medium for growing skins. Skins are not growing, only a very thin layer appears on top of the container. The result after folding the skin in 4-fold and after drying is a thin pellicule.
-http://fabtextiles.org/
+Indigo => skin has to be thick enough for staying 1 day in the indigo colour bath and still be flexible after drying. If skin is too thin at the start, than it becomes very brittle after colouring and drying.
-{{gallery>:learning-from-nature:bioshades}}
+Kurkuma => nearly no colouring when added to the growth medium
-=====dyeing with bacteria=====
+Hibiscus => enhances the growth of the skin in the medium
-     *  ±50×50 cm of silk chiffon or ready to dye cotton fabric
+Campèche +> enhances the growth of the skin in the medium
-  *    //Janthinobacterium lividum//
+Methods of coloring (in growth medium or coloring after growing)
-  *     glycerine (a couple of drops)
+Drying methods (open air, oven, ...)
-  *     alcohol
+Protection (coconut oil, Ricinus oil, bees wax, ...)
-  *     LB broth (lennox)
-  *     petri dishes
+recipe for mordanting skins, fabric or wool before dyeing with organic dyes:
-  *     inoculator loop
+for 40gr dry material (± 1 middlesized skin): 4gr alum, 2gr cream of tartar, 10gr Alkanet seeds soaked beforehand in alkohol to extract the color. Leave the material to boil in this bath for 1 hour. Rince and dry. The skins have to be thick enough, otherwise they become very brittle after drying.
-  *     camping gas+holder
-  *     incubator
-  *     gloves
-  *     autoclave bags for sterilisation
-  *     500 ml glass bottle with lid
-  *     cooking stove
-  *     pressure cooker / autoclave
-Bacteria killing:
-  * Attached the step by step procedure to inoculate new plates but also growing them on textiles.
-  * How to make the medium if you don't have it ready made: http://biohackacademy.github.io/bha4/cultivation-media/nutrient-agar/
-  * To kill the bacteria after use: * wear gloves * take all textiles out of petri dishes and place them in an autoclave bag * take all petri dishes and place them into a bag * put enough water in the pressure cooker, if you use the same pressure cooker i guess ±10 cm * close it, boil 10 min * turn it off leaving pot closed until the steam is gone * throw away autoclave bag with petri dishes * wash all the textiles with washing up liquid
-http://wiki.textile-academy.org/bootcamp2017bcn/handson/textilebacteria
 \\
-=====bioplastics=====
+=====organic dyes and bioplastics=====
-{{gallery>:learning-from-nature:bioplastics_dyes}}
+{{gallery>:learning-from-nature:bioplastics_dyes?lightbox}}
 Working with molds (silicone, pos/neg)
 Different flexibilities =>
@@ Line 59: / Line 39: @@
 Organic coloring and other additives
 \\
-{{:learning-from-nature:bioplastics_dyes}}
-=====organic dyes=====
-{{gallery>:learning-from-nature:skin_dyes}}
-Madder
-Alkanet
-Indigo
-Kurkuma
-Hibiscus
-Campèche
-Methods of coloring (in growth medium or coloring after growing)
-Drying methods (open air, oven, ...)
-Protection (coconut oil, Ricinus oil, bees wax, ...)
-\\
-=====tattooing skins=====
-{{gallery>:skins:tattoos}}
-Different experiments with tattooing on wet and dry skin.
-Handpoked tattoo with vegetal ink
-Experimental needle tattoo with self-made indigo ink
-Writing with watercolour marker