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dyeing_with_bacteria [2018/04/25 21:14] – [dyeing cellulose skin samples with Janthinobacterium lividum] annemie | dyeing_with_bacteria [2022/04/26 21:23] (current) – [Fermentation and probiotic bacteria] ami |
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====== dyeing with bacteria ====== | ===== pigmented bacteria ===== |
Conference non-human agents, art laboratory Berlin (2017) | Conference non-human agents, art laboratory Berlin (2017) |
http://www.artlaboratory-berlin.org/html/de-event-40.htm | http://www.artlaboratory-berlin.org/html/de-event-40.htm |
Bacterial dyes in Fashion: | Bacterial dyes in Fashion: |
https://www.asm.org/index.php/general-science-blog/item/6929-bacterial-dyes-in-fashion | https://www.asm.org/index.php/general-science-blog/item/6929-bacterial-dyes-in-fashion |
| https://issuu.com/kukkadesign/docs/living_colour-ibook |
Janthinobacterium lividum (Indigo): | Janthinobacterium lividum (Indigo): |
https://en.wikipedia.org/wiki/Janthinobacterium_lividum | https://en.wikipedia.org/wiki/Janthinobacterium_lividum |
Living Color: Bacterial Pigments | Living Color: Bacterial Pigments |
http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1000510 | http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1000510 |
| [[https://microbewiki.kenyon.edu/index.php/Streptomyces_coelicolor|Streptomyces coelicolor]], [[https://www.faberfutures.com/projects/project-coelicolor/colour-coded/|faber futures]] |
| Streptomyces have a life cycle similar to that of fungi. The cycle starts with growth of vegetative mycelium from a spore, followed by ariel mycelium, and, then, spores. |
| [[https://www.faberfutures.com/projects/project-coelicolor/scale-void-print/|Faber Futures - textile dyeing with bacteria]] |
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| Bacteria are everywhere.They create living pieces of art, e.g. the Rock Paintings : bacteria keep their colors alive. |
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| Pieces of art where the bacteria MAKE the piece (the design, the colors, the structure). |
| This can be induced/controlled/manipulated via nutrition and monitoring of temperature/humidity conditions. Many bacteria and Lichens get their food from the air, and following they create: |
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| - Reflection, light dispersion |
| - Luster, absorption, grating, polarisation |
| - Patterns, changing colours (mix different strains of bacteria to become different colours) |
| - Patterns will differ relating to different matrixes, variable growth medium |
| - Chromatics, optics |
| - The study of materials |
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| Cyanobacteria are the beginning of life. They are oxygen producing. |
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| Bioplastics with bacteria: |
| - With seaweed /agar agar (as a substitute for gelatine) |
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=====growing Janthinobacterium lividum===== | =====growing Janthinobacterium lividum===== |
{{gallery>:learning-from-nature:bacteria_skin }} | {{gallery>:learning-from-nature:bacteria_skin }} |
=====dyeing cellulose skin samples with Janthinobacterium lividum===== | =====dyeing cellulose skin samples with Janthinobacterium lividum===== |
{{gallery>:bacteria:samples }} | {{gallery>:bacteria:samples }} |
In the past (2017) I was succesfully growing colonies of //Lactobacillus plantarum// on cellulose skin drenched with LB medium. The biofilm with these bacteria was then used as a pollution sensor in the project Intelligent Guerilla Beehive, see the researchpage [[http://urbanbeelab.okno.be/doku.php?id=grow_your_own_beehive|grow your own beehive]]. Growing now //Janthinobacterium lividum// in the incubator, I wanted to research if this violet-blue bacteria will also thrive on microbial-skin-with-food. | In the past (2017) I was succesfully growing colonies of //Lactobacillus plantarum// on cellulose skin drenched with LB medium. The biofilm with these bacteria was then used as a pollution sensor in the project Intelligent Guerilla Beehive, see the researchpage [[http://urbanbeelab.okno.be/doku.php?id=grow_your_own_beehive|grow your own beehive]]. |
| Growing now //Janthinobacterium lividum// in the incubator, I wanted to research if this violet-blue bacteria will also thrive on microbial-skin-with-food. |
While growing the J.lividum bacteria in the petri's I found already out that they need some drops of glycerol to produce their blue color. So I made some LB growth medium with glycerol and poured it on the samples in the petris dishes, and I inoculated the 2 samples of fresh microbial skin with the J.lividum bacteria. After 2 days I saw yet a blu-ish shine on the wet white skin. I took them out of the incubator and poured some more medium. In one of the samples I injected some medium with a seringue and needle (photo 8). The next day I saw a development inside the skin-sample, but it was not clear if this was the bacteria multiplying or if it was mold. The next days it became clear that it was mold. Probably the needle was not completely sterile when I did the injection. The first sample became more and more blue, and under the microscope it was clear that the colony propagated themselves over the skin. The problem is that they constantly need food (LB) to keep them alive. | While growing the J.lividum bacteria in the petri's I found already out that they need some drops of glycerol to produce their blue color. So I made some LB growth medium with glycerol and poured it on the samples in the petris dishes, and I inoculated the 2 samples of fresh microbial skin with the J.lividum bacteria. After 2 days I saw yet a blu-ish shine on the wet white skin. I took them out of the incubator and poured some more medium. In one of the samples I injected some medium with a seringue and needle (photo 8). The next day I saw a development inside the skin-sample, but it was not clear if this was the bacteria multiplying or if it was mold. The next days it became clear that it was mold. Probably the needle was not completely sterile when I did the injection. The first sample became more and more blue, and under the microscope it was clear that the colony propagated themselves over the skin. The problem is that they constantly need food (LB) to keep them alive. |
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=====workshop bioshades Barcelona - 15/3/2018===== | =====workshop bioshades Barcelona - 15/3/2018===== |
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{{url>https://www.youtube.com/embed/gJvxqujkUvI?rel=0#?embed=true 560px,315px noborder}} | |
http://docs.academany.org/softacademy-handbook/_book/classes/00_intro.html | |
http://fabtextiles.org/ | http://fabtextiles.org/ |
{{gallery>:learning-from-nature:bioshades }} | {{gallery>:learning-from-nature:bioshades }} |
* cooking stove | * cooking stove |
* pressure cooker / autoclave | * pressure cooker / autoclave |
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| =====alternative biohacker recipe for nutrient medium for growing bacteria===== |
| __Nutrient Agar (NA)__ : Suitable for growing a wide range of bacteria none selectively |
| Compound Amount Unit |
| Yeast extract 2.0 g |
| Peptone 5.0 g |
| NaCl 5.0 g |
| Agar 15.0 g |
| Demi water up to 1000 mL |
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| Set pH ~4.7 |
| Please note: peptone is very dusty, so try not to inhale it. |
| Peptone can be found on eBay. |
| Yeast extract is often sold as pills in drugstores |
| Peptone can be replaced by any peptic digest of animal tissue |
| Yeast extract can be replaced by beef extract |
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=====killing bacteria ===== | =====killing bacteria ===== |
http://wiki.textile-academy.org/bootcamp2017bcn/handson/textilebacteria | http://wiki.textile-academy.org/bootcamp2017bcn/handson/textilebacteria |
https://livingcolour.eu/ | https://livingcolour.eu/ |
| {{ :dyeing:biodyes_general.pdf |bio-shades}} |
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