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hydrophobic_bc [2020/03/09 09:55]
ami [Protocol]
hydrophobic_bc [2020/03/09 10:34]
ami [Brussels Tests]
Line 30: Line 30:
 =====Brussels Tests===== =====Brussels Tests=====
 +Test1 (Brussel, 25/2/2020): Nostoc; Nostoc + Lichen
 {{gallery>:hydrophobicbc:test1brussel-nostoc}} {{gallery>:hydrophobicbc:test1brussel-nostoc}}
-Test1 (Brussel, 20/2/2020): Nostoc; Nostoc + Lichen 
 +Test1 (Brussel, 25/2/2020): Synechocystis; Synechocystis + Lichen
 +I followed the protocol we discussed,
 +- I took a nice piece of BC that I’ve cut in pieces of 20 grs each.
 +- I neutralised the BC as prescribed in the protocol, and I autoclaved the little pieces, together with a bit of BG11 still in the bottles.
 +- I’ve put the pieces in sterile petri dishes (working with the flame) and I inoculated them with Nostoc & Synechocystis (separately), on some petri's I added cleaned lichen on top of the cyanobacteria.
 +- Everytime I made 2 petri’s, to have a control. 
 +- I’ve put the petri's in the incubator at 22°C, on 25/2/2020.
 +- When I came back from Paris and when I controlled the petri’s, there was the same mould on all BC pieces.
 +This kind of ‘greenish blue cheese penicilline mould’. On the samples inoculated with Synechocystis, there was besides the blue/green mould also a kind of black mould.
 +- The samples with cyanobacteria + lichen had more mould than the ones without lichen. The samples inoculated with Synechocystis had more mould than the samples with Nostoc. The little black pieces on top of the BC on the petri’s with ‘lichen’ samples, are the lichen.
 +- The contaminated samles are not yet tested on their pH, neither I have inspected the (possible) growth and the mould under the microcope. This needs to be done asap.
hydrophobic_bc.txt · Last modified: 2020/03/09 10:35 by ami